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KMID : 0378019860290070057
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New Medical Journal
1986 Volume.29 No. 7 p.57 ~ p.62
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Effect of Ethanol on Na-K-ATPase Activity of Rabbit Kidney
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Abstract
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In order to determine the effect of ethanol on Na-K-ATPase and to clarify the action mechanisms of ethanol, the effects of n-alcohols including ethanol were observed in rabbit kidney homogenate. Na-K-ATPase activity was inhibited by ethanol in higher concentrations than 1.0%, in dose-dependent manner, but Mg-ATPase activity was affected in only 10 and 15% concentrations.
The effects of ethanol on Na-K-ATPase activity were unaffected by preincubation time and the enzyme concentrations. For the n-aliphatic alcohols, there exists a linear relationship between the logarithm of equiactive concentrations and the number of carbon atms in the aliphatic chain.. Arrhenius plot showed two break points at 20° and 30°C in the control medium which decreased to 18° and 28°C, respectively, by 7% ethanol.
These results indicate that ethanol inhibited Na-K-ATPase activity by interacting with the hydrophobic region and resulting in a disturbance of microenvironment of lipids associated with the enzyme. In addition, the hydrophobic bonds are shown to have an important role in maintaining the Na-K-ATPase activity.
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